Bio-speedy SARS CoV-2 Triple Gene RT-qPCR
Swab samples should be collected using Dacron or Polyester swabs. Other specimen types should be transferred in sterile containers. In the transport phase, Viral Transport Medium (VTM) (Preperation of viral transport medium.
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Bio-speedy SARS CoV-2 Triple Gene RT-qPCR
Bio-speedy SARS CoV-2 Triple Gene RT-qPCR Table 1. Kit content [Shelf Life: 18 months; refer to the expiration date on the box. Each reagent stored at storage temperature, may be used until the expiration date indicated on the tube. The expiration date of the kit is determined by the expiration date of the reagents].
| Storage Temperature: -20°C, Transport Temperature: +2-8°C | ||||||
| Content/Intended Use | Content | Quantity (10 p.L Reactions) | Consumptio n / Reaction | |||
| 100 Rxns | 250 Rxns | 500 Rxns | 1000 Rxns | |||
| SARS-CoV-2 (RdRp), SARS-CoV-2 (N) and
SARS-CoV-2 (ORF1ab) genes (FAM) |
CVD Tri Oligo Mix | 1 x 250 uL | 1 x 625 uL | 1 x 1250 uL | 2 x 1250 uL | 2.5 uL |
| Internal Control (IC) (RNase P) (HEX) | ||||||
| DNA polymerase, dNTP mix, reaction buffer, reverse transcriptase and ribonuclease inhibitor | 2X Prime Script Mix | 1 x 500 uL | 1 x 1250 uL | 2 x 1250 uL | 4 x 1250 uL | 5 uL |
| Storage Temperature: +2-8°C/-20°C; Transport Temperature +2-8°C/-20°C
If the components are frozen store at -20°C, it should be stored at 2-8 °C without being frozen again after thawing. |
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| Negative Control Template
Test it in each run for contamination control |
NTC | 1 x 1000 uL | 1 x 1000 uL | 1 x 1000 uL | 1 x 1000 uL | 2.5 uL |
| Positive Control Template: Synthetic SARS- CoV-2 and RnaseP genom fragment
Test it in each run for reactive stability control |
PC CVD Tri | 1 x 250 uL | 1 x 250 uL | 1 x 500 uL | 2 x 500 uL | 2.5 uL |
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